Fig. 3.

Flow cytometry analysis of α1,2-fucosylated epitopes in HNSCC panel. A panel of cell lines including two control cell lines, NOK and DOK, and eight HNSCC cell lines spanning multiple HNSCC subsites, including A-253 salivary gland carcinoma cells, FaDu and Detroit-562 (Det-562) pharyngeal squamous carcinoma cells, and CAL-27, SCC-9, SCC-15, SCC-25 and HSC-3 tongue squamous carcinoma cells, was analyzed via flow cytometry for three α1,2-fucosylated epitopes, including (A) H antigen, (B), Lewis Y (Le^Y) and (C) Lewis B (Le^B). For all flow cytometry experiments, unstained controls and isotype controls were performed. Mean fluorescence intensity is reported in relative fluorescence units (technical replicates, n = 3).