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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Mol Neurobiol. 2022 Mar 16;59(5):3233–3253. doi: 10.1007/s12035-022-02747-0

Figure 8. nSMase2 deficiency reduces the number of exosomes that decrease Grin2b.

Figure 8.

(A) The upstream regulator analysis by IPA showing transcripts downstream of REST and miR-223-3p that is decreased in fro/fro cortex. Inhibition of REST and miR-223-3p (blue) activate Grin2b. The transcripts downstream of REST and miR-223-3p are positively associated with neuron number, long-term potentiation, epilepsy, behavioral conditioning, and memory. (B) qPCR for miR-223-3p in exosomes from fro/fro and +/fro brain. Plot shows fold-change when compared to exosomal house keeping miRNA (miR-30c-5p). N=3, unpaired t-test. (C, D) Nanoparticle tracking analysis (Zetaview) of extracellular vesicles from +/fro and fro/fro cortex. The mumber of exosomes are shown as per militer (ml) of PBS or per gram (gm) of brain tissues N=3. Unpaired t-test. P < 0.01 (C) and 0.05 (D).