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. 2022 Apr 7;9:853409. doi: 10.3389/fnut.2022.853409

Figure 3.

Figure 3

High-fat diets increase intestinal permeability and then weaken the interception of LPS. (A) Parameters of intestinal permeability: pasma FITC-d, D-LA and DAO. Plasma FITC-d was measured at 4 hours after oral administration of (900 mg/kg) FITC-d. (B) Assessment of LPS penetration into the circulation. Real-time fluctuation of plasma LPS concentration is monitored within 1–5 h after oral administration of (1 mg/kg) LPS. (C) Observation of LPS migration in the intestine using confocal laser at 5 hours after oral administration of (1 mg/kg) FITC-LPS. (D) Analysis of FITC-LPS concentration in plasma using fluorescence microplate reader for at 5 h after oral administration of (1 mg/kg) FITC-LPS. (E) Histology analysis of intestinal by hematoxylin-eosin (HE) staining. (F) Histology analysis of goblet cells by periodic acid-schiff (PAS) stain. (G) The number of goblet cells was counted with a flow cytometer. Data are shown as mean ± SEM. N = 4. Two-tailed unpaired Student's t-test was used. *P < 0.05, **P < 0.01, ***P < 0.001; One-way ANOVA was used. Diverse letters show significant differences (P < 0.05).