Table 2.
The linear regression analyses for the calibration curves data and sensitivity for D3 and its major metabolites in human serum using the developed UHPLC-ESI-MS/MS method.
| Analyte | Calibration curvea (n = 5) |
LLOQb (ng mL−1) | |||
|---|---|---|---|---|---|
| Range (ng mL−1) | Slopeb (±SD) | Interceptb (±SD) | r | ||
| D3 | 0.05–50 | 1.18 (±0.05) | 0.056(±0.002) | 0.9991 | 0.05 |
| 25(OH)D3 | 0.02–50 | 1.54 (±0.06) | 0.024 (±0.002) | 0.9990 | 0.02 |
| 1,25(OH)2D3 | 0.02–50 | 1.69 (±0.07) | 0.019 (±0.001) | 0.9987 | 0.02 |
a
Peak area ratio of D3, 25(OH)D3 or 1,25(OH)2D3 and I.S. versus serum concentration (ng mL−1).
b
Data presented as mean (n = 5) ± SD.