Table 4.
Extraction recovery and matrix effect of D3 and its major metabolites from human serum after SALLE and assay using the developed UHPLC-ESI-MS/MS method.
| Analyte | Concentration (ng mL−1) | Extraction recoverya (%) ± S.D. | Matrix factora (IS-normalized) |
|---|---|---|---|
| D3 | 0.05 | 95.9 ± 4.6 | 0.955 |
| 5 | 98.7 ± 3.0 | 0.984 | |
| 50 | 99.3 ± 1.9 | 1.004 | |
| 25(OH)D3 | 0.05 | 96.1 ± 4.1 | 0.975 |
| 5 | 97.7 ± 2.7 | 0.988 | |
| 50 | 100.4 ± 1.9 | 0.999 | |
| 1,25(OH)2D3 | 0.05 | 96.9 ± 4.8 | 0.979 |
| 5 | 99.1 ± 2.9 | 0.990 | |
| 50 | 101.0 ± 1.6 | 1.007 |
a
Average of five determinations.