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. 2021 Dec 22;50(7):e40. doi: 10.1093/nar/gkab1258

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Specificity of the PAFN in live cells. (A) Confocal fluorescence images of MCF-7 cells, A-549 cells and 7721 cells incubated with PAFN under UV light irradiation. (B) Normalized FRET efficiency value is quantified by ImageJ in three cell lines. (C) Confocal fluorescence images of MCF-7 cells treated with let-7a inhibitor or let-7a mimic by lipofectamine™ 3000 transfection reagent for 3 h in advance, followed by incubation with PAFN under UV light irradiation. (D) Normalized FRET efficiency value is quantified by Image J in MCF-7 cells treated with different condition. Values are mean ± SD (n = 3), statistical significance was determined by two-tailed Student's t-test, the symbol ** indicates the statistical significance at levels of P < 0.01. Scar bar is 20 μm.