Table 2.
Oligonucleotides used in this study with predicted number of G-tetrad stacks based on CD 264 nm amplitude. See Supplementary Table S2 for sequences used in SAXS Rg analysis
| ODN Name | Sequence (5′→ 3′) | # nt | # G-Stacks | ϵ (M–1 cm–1) | MW (Da) (strand) | CD Δϵ264 (M–1 cm–1) | Predicted # G-stacks |
|---|---|---|---|---|---|---|---|
| TG3T | TGG GT | 5 | 3 | 471,500 | 1534 | ||
| TG4T | TGG GGT | 6 | 4 | 576,200 | 1863 | ||
| TG5T | TGG GGG T | 7 | 5 | 680,900 | 2193 | ||
| TG6T | TGG GGG GT | 8 | 6 | 785,600 | 2522 | ||
| 1XAV | TGA GGG TGG GTA GGG TGG GTA A | 22 | 3 | 228,700 | 6992 | 190 | 2.9 |
| 2LBY | TAG GGA GGG TAG GGA GGG T | 19 | 3 | 201,700 | 6054 | 206 | 3.1 |
| 2M27 | CGG GGC GGG CCT TGG GCG GGG T | 22 | 3 | 200,400 | 6905 | 220 | 3.2 |
| 5I2V | AGG GCG GTG TGG GAA TAG GGA A | 22 | 3 | 233,100 | 6970 | 182 | 2.8 |
| c-Myc-8 | GGG GAG GGT GGG GAG GGT GGG GAA GGT GGG GAG G | 34 | 354,900 | 10976 | 361 | 4.7 | |
| c-Myc-12 | GGG AAC CCG GGA GGG GCG CTT ATG GGG AGG GTG GGG AGG GTG GGG AAG GTG GGG AGG AGA CTC AGC CGG G | 70 | 702,100 | 22255 | 705 | 8.3 | |
| c-Kit-8 | GGG CGG GCG CGA GGG AGG GGA GGC GAG GGG CGT GG | 38 | 381,800 | 12143 | 317 | 4.2 | |
| c-Kit-12 | GGG CGG GCG CGA GGG AGG GGA GGC GAG GGG CGT GGC CGG CGC GCA GAG GGA GGG CGC TGG G | 64 | 626,800 | 20349 | 339 | 4.5 | |
| k-Ras-8 | GGG AGC GGC TGA GGG CGG TGT GGG AAG AGG GAA GAG GGG GAG G* | 43 | 445,300 | 13755 | 276 | 3.8 |
*Underlined region of k-Ras-8 overlaps with sequence studied in ref (76).