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. 2022 Mar 31;50(7):4012–4028. doi: 10.1093/nar/gkac184

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — i⁶A modification is a prerequisite for m³C32 biogenesis in hmtRNA^Ser(UCN) by METTL8-Iso1. (A) Time-course curves of the m³C32 modification of hmtRNA^Ser(UCN) and i⁶A-hmtRNA^Ser(UCN) by METTL8-Iso1. Negative controls (no tRNA) represent assays without tRNA added. (B) EcMiaA was able to efficiently modify hmtRNA^Ser(UCN). (C) UPLC–MS/MS analysis of the digested product of EcMiaA-modified hmtRNA^Ser(UCN). m/z value 268.2 to 136.2 (A) and m/z value 336.2 to 204.1 (i⁶A) are shown on the right. Data represent averages of three (A) or two (B) independent experiments and the corresponding SEM.