Figure 1.

The overall outline of the presented study. We examined the on-target activity problem for CRISPR–Cas9. Features affecting cleavage efficiency are initially described, followed by predictive tools to assist in the guide design process. These tools are then evaluated on six datasets from various organisms (i.e. human, mouse, zebrafish) using two metrics, Spearman correlation and normalized discounted cumulative gain (nDCG). Tasks and tools not examined in the study are colored grey. Examples of features, tools, and datasets are provided in parentheses.