REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CR3022	Meulen et al., 2006	DQ 168569.1, DQ168570.1
HRP-conjugated goat anti-human IgG	Southern Biotech	Cat#2040-05; RRID: AB_2795644
Anti-human CD45-APC-Alexa Fluor 700	BioLegend	Cat#304024; RRID: AB_493761
Anti-human CD3-BV605	BD Biosciences	Cat# 563217; RRID: AB_2714001
Anti-human CD19-Brilliant Violet 605	BioLegend	Cat# 302244; RRID: AB_2562015
Anti-human CD56-Brilliant Violet 605	BioLegend	Cat# 362538; RRID: AB_2565856
Anti-human HLA-DR-PE/Cy7	BD Biosciences	Cat# 560651; RRID: AB_1727528
Anti-human CD11b-FITC	BioLegend	Cat# 301330; RRID: AB_2561703
Anti-human CD33-PE	BioLegend	Cat# 366608; RRID: AB_2566107
Anti-human CD14 BV421	BD Biosciences	Cat# 565283; RRID: AB_2739154
Anti-human CD15-APC	BioLegend	Cat# 323007; RRID: AB_756013
Anti-human CD45-BV570	BD Biosciences	Cat# 304034; RRID: AB_2563426
Anti-human CD3-BUV661	BD Biosciences	Cat# 741596; RRID: AB_2871005
Anti-human CD4-PE/Cy5	BD Biosciences	Cat# 555348; RRID: AB_395753
Anti-human CD8-BB660	BD Biosciences, custom	Cat#624295; no RRID
Anti-human CD-BV786	BD Biosciences	Cat# 563823; RRID: AB_2687487
Anti-human CD19-BUV563	BD Biosciences	Cat# 612916; RRID: AB_2870201
Anti-human CD56 BB515	BD Biosciences	Cat# 564488; RRID: AB_2744428
Anti-human HLA-DR-APC/H7	BD Biosciences	Cat# 561358; RRID: AB_10611876
Anti-human CD16-BUV395	BD Biosciences	Cat# 563784; RRID: AB_2744293
Anti-human CD11c-APC-R700	BD Biosciences	Cat# 566610; RRID: AB_2869792
Anti-human CD88-PE/Cyanine7	BioLegend	Cat# 344308; RRID: AB_11126750
Anti-human CD1c-BUV737	BD Biosciences	Cat# 748723; RRID: AB_2873127
Anti-human CD123-BUV496	BD Biosciences	Cat# 564196; RRID: AB_2738662
Anti-human CD141-BV605	BD Biosciences	Cat# 740421; RRID: AB_2740151
Anti-human CD5-BV480	BD Biosciences	Cat# 566122; RRID: AB_2739524
Anti-human CD163-PE/CF594	BD Biosciences	Cat# 562670; RRID: AB_2737711
Anti-human CD163-BV786	BD Biosciences	Cat# 741003; RRID: AB_2740626
Anti-human Siglec-6-BV650	BD Biosciences	Cat# 747911; RRID: AB_2872373
Anti-human Axl-BUV615	BD Biosciences	Cat# 751050; RRID: AB_2875090
Anti-human CD45-BUV805	BD Biosciences	Cat# 612891; RRID: AB_2870179
Anti-human CCR2-Brilliant Violet 785	BioLegend	Cat# 150621; RRID: AB_2721565
Anti-human CXCR3-PE/Cyanine5	BioLegend	Cat# 353755; RRID: AB_2904375
Anti-human CD86-PE	BD Biosciences	Cat# 555665; RRID: AB_396019
Anti-human CD69-BV711	BD Biosciences	Cat# 563836; RRID: AB_2738443
Annexin V Alexa Fluor™647 conjugated	Thermo Fisher Scientific	Cat# A23204; RRID: AB_2341149
Briliant Violet 785 Mouse IgG2a, κ Isotype control	BioLegend	Cat#400647; no RRID
PE/Cyanine5 Mouse IgG1, κ Isotype control	BioLegend	Cat#400118; no RRID
Human TruStain FcX (Fc receptor blocking solution)	BioLegend	Cat#422302
Bacterial and virus strains
VSV pseudovirus bearng SARS-CoV-2 spike protein	Tani et al., 2021	N/A
Biological samples
Human venous blood	Tokyo-Shinagawa Hospital	N/A
Chemicals, peptides, and recombinant proteins
RBD (amino acids: 331-529)	In-house	MN994467
HiTrap Protein G HP Columns	Cytiva	17-0404-01
Tween-20	Fujifilm	167-11515
Can Get Signal #2	TOYOBO	NKB-301
OPD substrate	Sigma-Aldrich	P8287
D-MEM (Low Glucose) with L-Glutamine and Phenol Red	Fujifilm Wako Pure Chemicals	041-29775
D-MEM (High Glucose) with L-Glutamine and Phenol Red	Fujifilm Wako Pure Chemicals	044-29765
Bovine serum albumin	Sigma-Aldrich	A2153
Fetal bovine serum	Biowest	S1780-500
Fetal bovine serum	Nichirei Biosciences	171012
Geneticin	Thermo Fisher Scientific	10131-027
Penicillin/streptomycin	Thermo Fisher Scientific	15140-122
Phosphate buffered saline (-)	Fujifilm Wako Pure Chemicals	166-23555
RPMI-1640 with L-glutamine and phenol red	Fujifilm Wako Pure Chemicals	189-02025
Penicillin-Streptomycin solution	Fujifilm Wako Pure Chemicals	168-23191
0.5M EDTA	Thermo Fisher Scientific	15575
Bovine serum albumin fraction V	Roche	10735086001
CELLBANKER 1 plus	ZENOAQ RESOURCE	CD021
Brilliant Stain Buffer Plus	BD Biosciences	566385
7-amino-actinomycin D	Sigma-Aldrich	A9400
Critical commercial assays
Bright-Glo luciferase assay system	Promega	E2620
Expi293 expression system	Thermo Fisher Scientific	A29133
Elecsys Anti-SARS-CoV-2	Roche Diagnostics	518316181
Cytometric Bead Array Human IL-1β	BD Biosciences	558279
Cytometric Bead Array Human IL-2	BD Biosciences	558270
Cytometric Bead Array Human IL-4	BD Biosciences	558272
Cytometric Bead Array Human IL-5	BD Biosciences	558278
Cytometric Bead Array Human IL-6	BD Biosciences	558276
Cytometric Bead Array Human IL-8	BD Biosciences	558277
Cytometric Bead Array Human IL-10	BD Biosciences	558274
Cytometric Bead Array Human IL-12p70	BD Biosciences	558283
Cytometric Bead Array Human IL-17F	BD Biosciences	562151
Cytometric Bead Array Human G-CSF	BD Biosciences	558326
Cytometric Bead Array Human GM-CSF	BD Biosciences	558335
Cytometric Bead Array Human IFN-α	BD Biosciences	560379
Cytometric Bead Array Human IFN-γ	BD Biosciences	558269
Cytometric Bead Array Human IP-10	BD Biosciences	558280
Cytometric Bead Array Human MCP-1	BD Biosciences	558287
Cytometric Bead Array Human MIG	BD Biosciences	558286
Cytometric Bead Array Human MIP-1α	BD Biosciences	558325
Cytometric Bead Array Human TNF-α	BD Biosciences	560112
Enhanced Sensitivity Cytometric Bead Array Human IFN-γ	BD Biosciences	561515
Enhanced Sensitivity Cytometric Bead Array Human IL-1β	BD Biosciences	561509
Enhanced Sensitivity Cytometric Bead Array Human IL-2	BD Biosciences	561517
Enhanced Sensitivity Cytometric Bead Array Human IL-4	BD Biosciences	561510
Enhanced Sensitivity Cytometric Bead Array Human IL-5	BD Biosciences	561511
Enhanced Sensitivity Cytometric Bead Array Human IL-6	BD Biosciences	561512
Enhanced Sensitivity Cytometric Bead Array Human IL-8	BD Biosciences	561513
Enhanced Sensitivity Cytometric Bead Array Human IL-10	BD Biosciences	561514
Enhanced Sensitivity Cytometric Bead Array Human IL- IL-12p70	BD Biosciences	561518
Enhanced Sensitivity Cytometric Bead Array Human IL- TNF-α	BD Biosciences	561516
Human Soluble Protein Master Buffer Kit	BD Biosciences	558265
Human Enhanced Sensitivity Master Buffer Kit	BD Biosciences	562248
Experimental models: Cell lines
VeroE6/TMPRSS2 cells	JCRB Cell Bank	JCRB1819
Software and algorithms
FACSDiva	BD Biosciences	https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-software/flow-cytometry-acquisition/facsdiva-software
FCAP Array Software Version 3.0	BD Biosciences	https://www.bdbiosciences.com/us/applications/research/bead-based-immunoassays/analysis-software/fcap-array-software-v30/p/652099
FlowJo v10.8.0	BD Biosciences	http://www.flowjo.com/
GraphPad Prism version 9	GraphPad Software, Inc.	https://www.graphpad.com/scientific-software/prism/
Python -Version 3.7.3	Python Software Foundation.	https://www.python.org/
UMAP (Python) - Version 0.5.2	Mclnnes et al., 2018	https://umap-learn.readthedocs.io/en/latest/
Other
BD Vacutainer® CPT™ Tube	BD Biosciences	362761
iMark microplate reader	Bio-Rad	168-1130J1
Eposh2	Biotek	EPOCH2NS
GroMax Navigator Microplate Luminometer	Promega	GM2000
Cobas e 411 plus	Roche Diagnostics	N/A