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. 2022 Apr 21;13:2174. doi: 10.1038/s41467-022-29850-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — HS-AFM images (Supplementary Movie 2) showing the polymerization of Snf7 on an undulated (concave-convex) nanopatterned support covered with a SLB: Raw data HS-AFM frames (a), merged support-channel (green) and Snf7 protein-channel (magenta) (b), separate Snf7 protein-channel (magenta) (c) and binarized protein-channel (d). Left: Substrate height (e), slope (f), and curvature (g) maps calculated from the support-channel. Middle: Binned histograms of height (e), slope (f) and curvature (g), respectively, with bin area proportional to the number of pixels of a given characteristic. Right: Unbinned occupancy of individual pixels by Snf7 (d) as a function of time (horizontal axis) and sorted by surface characteristic height (e), slope (f) and curvature (g). Each row represents an individual pixel, and each column represents a timepoint. h Schematic of spherical cap fitting procedure for surface curvature calculation. A sphere is fit to a ring of pixels with a user-defined radius (r, in this case, 15 pixels) from the central pixel. The radius of the fitted sphere (R_c) is determined from the ring radius (r) and the difference (z) between the mean height value of the pixels within the ring (green dot) and the height of the central pixel (magenta dot). The example shown in h corresponds to the outline in e. i Snf7 surface occupancy as a function of time, shown as percentage of the total frame pixel number. j HS-AFM visualization of spiral growth: the filament grows at the spiral periphery (Supplementary Movie 3). Source data are provided as a Source Data file.