Fig. 6. MADCAM1 mutations are involved in immunosuppression.

A The survival rate of MADCAM1^WT, MADCAM1^MUT and control GC cells after co-culture with activated PBMC. B The relative expression of CD274 (PD-L1) in MADCAM1^WT, MADCAM1^MUT and control GC cells. C The survival rate of MADCAM1^WT, MADCAM1^MUT and control GC cells after co-culture with activated PBMC in the absence or presence of anti-PD-1 antibodies. D Diagrammatic representation of reprogramming macrophage by co-culture of GC cell with PMA treated THP-1. E Strong CD163 by western blot were observed in MADCAM1^P270Q-reprogrammed TAMs and MADCAM1^D242N-reprogrammed-TAMs. F Migration assays of THP1 macrophage by the supernatants from corresponding reprogrammed TAMs, M0 or M2 macrophages. G The migration assays of AGS in the absence or presence of corresponding reprogrammed TAMs. H The NOD/SCID mice (5 mice/group) co-injected subcutaneously with MGC-803 cells and blank control or MADCAM1^WT-reprogrammed TAM or MADCAM1^D242N -reprogrammed TAM. After 2 months, the mice co-injected with MADCAM1^D242N -reprogrammed TAM and MGC-803 cells have more area of lung metastatic loci. I The survival rate of GC cells after co-culture with activated PBMC along with corresponding reprogrammed TAMs. J The survival rate of GC cells after co-culture with activated PBMC along with corresponding reprogrammed TAMs in the absence or presence of anti-PD-1 antibodies. Bars, ±SD; *p < 0.05; **p < 0.01. ***p < 0.001.