Figure 3.
Frequency of monocyte subsets in HICs and control groups. (A) Frequencies of classical (CM; CD14++CD16−), Intermediate (IM; CD14++CD16+) and non-classical monocytes (NCM; CD14+CD16++) in unstimulated samples; (B) Frequencies of CM, IM, and NCM in LPS-stimulated samples; (C) Significant Spearman correlations between frequency of unstimulated CM, IM and NCM and other markers evaluated in the study; Colored horizontal bars represent the IQR and sample median, while gray boxplots represent linear model estimated marginal sample-bias adjusted means and 95% confidence intervals (CI 95%). Comparisons of estimated marginal means among groups were performed by T-tests of contrasts obtained after multivariate-linear models fitted by ordinary least square regressions. p-values were corrected by the Tukey Honest Significant Difference post hoc method and represented as: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Comparison graphs were plotted with Graphpad Prism v9 and correlation graphs were plotted with R software v4.1.