Table 1.
Cytotoxic potential of selenocompounds against sensitive ovarian (HOC) and breast carcinoma (MCF-7) cell lines and their Adriamycin (ADR) and paclitaxel (PAX) resistant sub-lines with the corresponding collateral sensitivity (CS) index.
| Cpd | HOC | HOC/ADR | CS | MCF-7 | MCF-7/PAX | CS |
|---|---|---|---|---|---|---|
| IC50 [µM] | IC50 [µM] | |||||
| K1 | 0.8 ± 0.0b | 2.3 ± 0.0a | 0.4 ± 0.0 | 1.6 ± 0.1b | 2.2 ± 0.2b,c,d,e | 0.7 ± 0.1 |
| K2 | 0.8 ± 0.1b | 2.4 ± 0.1a | 0.3 ± 0.0 | 1.9 ± 0.1b,c,d | 1.9 ± 0.1b,c | 1.0 ± 0.1 |
| K3 | 1.8 ± 0.0c | 2.6 ± 0.1a | 0.7 ± 0.1 | 2.0 ± 0.5b,c,d,e | 2.1 ± 0.1b,c,d | 1.0 ± 0.3 |
| K4 | 2.2 ± 0.1c,d | 3.6 ± 0.1b | 0.6 ± 0.0 | 1.8 ± 0.1b,c,d | 3.3 ± 0.3h | 0.6 ± 0.0 |
| K5 | 1.1 ± 0.0b | 2.2 ± 0.3a | 0.5 ± 0.1 | 1.6 ± 0.1b,c | 2.8 ± 0.1g | 0.6 ± 0.1 |
| K6 | 0.7 ± 0.1b | 2.6 ± 0.1a | 0.3 ± 0.0 | 2.1 ± 0.2b,c,d,e | 2.3 ± 0.3c,d,e | 0.9 ± 0.1 |
| K7 | 1.1 ± 0.1b | 3.1 ± 0.3a,b | 0.4 ± 0.1 | 1.9 ± 0.1b,c,d | 2.4 ± 0.1d,e,f | 0.8 ± 0.1 |
| K8 | 2.1 ± 0.1c,d | 2.7 ± 0.0a | 0.8 ± 0.1 | 2.6 ± 0.4e,f | 2.0 ± 0.1b,c | 1.3 ± 0.3 |
| N1 | 2.7 ± 0.3e,f | 4.2 ± 0.2d | 0.7 ± 0.1 | 3.1 ± 0.2f | 2.5 ± 0.0e,f,g | 1.2 ± 0.2 |
| N2 | 7.1 ± 0.2h | 4.7 ± 0.1c | 1.5 ± 0.1 | 2.6 ± 0.1e,f | 2.5 ± 0.3e,f,g | 1.0 ± 0.1 |
| N3 | 6.5 ± 0.3g | 5.2 ± 0.1c,d | 1.3 ± 0.1 | 2.3 ± 0.5c,d,e | 2.7 ± 0.2f,g | 0.9 ± 0.3 |
| N4 | 2.4 ± 0.1d,e | 3.7 ± 0.2b | 0.6 ± 0.1 | 2.1 ± 0.1b,c,d,e | 2.4 ± 0.2d,e,f | 0.9 ± 0.1 |
| N5 | 2.3 ± 0.1d | 4.5 ± 0.2c | 0.5 ± 0.1 | 2.5 ± 0.2d,e,f | 2.7 ± 0.2f,g | 0.9 ± 0.2 |
| N6 | 2.4 ± 0.2d,e | 3.6 ± 0.0b | 0.7 ± 0.1 | 1.7 ± 0.0b,c | 1.9 ± 0.1b | 0.9 ± 0.0 |
| N7 | 2.9 ± 0.1f | 5.7 ± 0.2d | 0.7 ± 0.0 | 2.2 ± 0.1b,c,d,e | 2.7 ± 0.3f,g | 0.8 ± 0.1 |
| ADR | 0.01 ± 0.0a | 2.5 ± 0.0a | 0.0 ± 0.0 | – | – | – |
| PAX | – | – | – | 0.03 ± 0.0a | 1.5 ± 0.0a | 0.0 ± 0.0 |
Data are expressed as the average inhibitory concentration (IC50) and collateral sensitivity index (CS) of three repetitions with standard error of the mean (SEM). The selectivity is considered as a strongly selective, if CS value is higher than 6, moderately selective if 6 > CS > 3, slightly selective if 3 > CS > 1 and non-selective if CS is less than 1. The statistical differences between compounds were calculated by one-way analysis of variance (ANOVA) and Duncan’s post hoc test (p ˂ 0.05) within one cell line, where different letters (a-h) denoted the significances; the different cell lines were evaluated independently on each other.
Significant values are in [bold].