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. 2022 Apr 21;13:2172. doi: 10.1038/s41467-022-29694-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Top: Hi-C normalized interaction matrix at 100 kb resolution for chromosomal scaffold 10. Bottom: Relative Hi-C count and TAD boundary scores as predicted by tadbit (1 = lowest, 10 = highest). b Violin plot of TAD size distributions for human and Euprymna scolopes computed at 100 kb resolution, plotted in 100 kb bins. c CTCF binding motif as identified by homer motif search in 100 kbp of predicted TAD boundaries. d Solvent area surface exposure (SASA) per bp for individual chromosomal scaffolds (observed and random). Conserved metazoan synteny on x-axis, cephalopod-specific microsynteny on y-axis. e Three-dimensional model of chromosomal scaffold 10 with novel (blue) and conserved (yellow) microsynteny locations labeled. Left and right models are the same, shifted by 90°.