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. 2022 Apr 8;10:868240. doi: 10.3389/fchem.2022.868240

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Copyright © 2022 Greule, Izoré, Machell, Hansen, Schoppet, De Voss, Charkoudian, Schittenhelm, Harmer and Cryle.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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UV–Visible spectroscopic binding studies of OxyA_kis and OxyC_kis with azole inhibitors. (A) Example for the spectral response of OxyA_kis (left) and OxyC_kis (right) upon different concentrations of clotrimazole. (B) Amplitudes of the binding of various azole inhibitors to OxyA_kis (left), OxyC_kis (right), and imidazole (C). Inhibitors tested: imidazole, miconazole, clotrimazole, ketoconazole, fluconazole, and itraconazole. (D) Summary of azole binding to OxyA_kis and OxyC_kis. ∆A_max and K_D derived from the one-site binding model. SE, standard error of the regression.