Generation of CCR5mut iPSCs from MCM fibroblasts and T cells
(A) Schematic diagram of the experimental design.
(B) Schematic representation showing the target site, sequences of the two gRNAs used to delete the 198-bp CCR5 fragment, and position of PCR primers (P1 and P2) used to detect deletion.
(C) Genomic PCR to detect deletion within CCR5 locus. ∗Denotes clones selected in these studies.
See also Figures S1, S2, and S4.