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. 2022 Mar 31;17(4):953–963. doi: 10.1016/j.stemcr.2022.03.003

Figure 1.

Figure 1

Generation of CCR5mut iPSCs from MCM fibroblasts and T cells

(A) Schematic diagram of the experimental design.

(B) Schematic representation showing the target site, sequences of the two gRNAs used to delete the 198-bp CCR5 fragment, and position of PCR primers (P1 and P2) used to detect deletion.

(C) Genomic PCR to detect deletion within CCR5 locus. Denotes clones selected in these studies.

See also Figures S1, S2, and S4.