Figure 2.

Generation of T cells from CCR5mut MCM iPSCs

(A) Schematic representation of hematopoietic differentiation of fib- and T-iPSCs to MHPs and their further differentiation to T cells.

(B) Both WT and CCR5mut iPSCs efficiently differentiated into MHPs as analyzed by flow cytometry of floating cells collected from iPSC/OP9 co-cultures on day 10 of differentiation.

(C) Day10 MHPs from WT and CCR5mut iPSCs were cultured on OP9-DLL4 in the presence of SCF, IL-7, and FLT3L for 2 weeks, and the floating cells were analyzed by flow cytometry after gating CD45⁺ cells. ^∗Intracellular CD3 was analyzed in WT and CCR5mut fib-iPSCs differentiation cultures.

In (B) and (C), control staining with the appropriate isotype matched mouse monoclonal antibody were included to establish a threshold for positive staining. The graphs are representative of at least three independent experiments.

See also Figures S3 and S4.