Figure 3.

GP2-enriched PPs have lower expression of endoderm-derived non-pancreatic cell markers

(A–C) RT-PCR quantification of SOX2, NKX2-1, and CDX2 normalized to RPL-19 housekeeping gene, in H1-derived NS, FT, and GP2⁺ PP fractions (n = 3 independent experiments, NS, FT, and GP2⁺; n = 1, human stomach, lung, and intestine; one-way ANOVA analysis with Tukey’s multiple comparisons; ^∗p < 0.05, ^∗∗∗p < 0.01; error bars represent SEM).

(D–G) Representative flow cytometry plots indicating SOX2 and CDX2 expression in NS, GP2⁻, and GP2⁺ populations and quantification of cell percentages (n = 7 independent experiments; one-way ANOVA analysis with Tukey’s multiple comparisons; ^∗p < 0.05, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001, error bars represent SEM).

See also Figures S4 and S5.