Figure 4.

GP2-enriched PPs are less proliferative and give rise to smaller grafts with lower CDX2⁺ and PRSS1⁺ cells

(A–D) Representative images and quantification of H&E and KU80 staining of the non-teratoma grafts retrieved from mice 15 weeks post-transplantation (scale bar is 400 μm; n = 3 independent experiments; one-way ANOVA analysis with Tukey’s multiple comparisons; ^∗p < 0.05, ^∗∗p < 0.01; error bars are SEM).

(E and F) RT-PCR quantification of MKI67 and TOP2A normalized to RPL19 housekeeping gene in NS, FT, and GP2⁺ populations before transplantation. (n = 3 independent experiments; one-way ANOVA analysis with Tukey’s multiple comparisons; ^∗p < 0.05, ^∗∗p < 0.01; error bars represent SEM).

(G and H) Representative flow cytometry plots indicating KI67 expression in NS, GP2⁻, and GP2⁺ populations (n = 7 independent experiments; one-way ANOVA analysis with Tukey’s multiple comparisons; ^∗p < 0.05, error bars represent SEM).

(I–Q) Representative immunostaining staining of grafts at week 15 post-transplantation, indicating the presence of cells expressing CDX2 (intestinal marker), NKX2-1 (lung marker), chromogranin A (CHGA, pancreatic endocrine marker), cytokeratin 19 (KRT19, ductal cell marker), and trypsin (PRSS1, acinar cell marker) (scale bar is 200μm), and quantification of cell numbers (n = 3 independent experiments; one-way ANOVA analysis with Tukey’s multiple comparisons; ^∗p < 0.05; error bars represent SEM).

(R) Ratio of endocrine to acinar cells (n = 3 independent experiments; error bars are SEM).