Figure 5.

GP2⁺-enriched PPs give rise to functional beta cells in vivo

(A and B) Glucose-stimulated C-peptide secretion assay at 15 weeks post-transplantation of H1- (A) or H9- (B) derived NS, FT, and GP2⁺ fractions ([A] NS, n = 15 mice from 6 independent experiments; FT, n = 14 mice from six independent experiments; GP2⁺, n = 9 mice from 6 independent experiments; [B] NS, n = 6 mice from three independent experiments; FT, n = 7 mice from 3 independent experiments; GP2⁺, n = 4 mice from three independent experiments; two-way ANOVA analysis with Tukey’s multiple comparisons; ^∗p < 0.05, ^∗∗p < 0.01; error bars represent SEM).

(C and D) Representative immunostaining staining of grafts without teratoma retrieved from mice at week 15 post-transplantation indicating the presence of cells expressing C-peptide (Cp, beta cell marker), glucagon (GCG, alpha cell marker), somatostatin (SST, delta cell marker), and SLC18A1 (enterochromaffin cell marker). DAPI marks cell nuclei. (Scale bar is 200μm).