Figure 1.

NMP-like cells are induced by intermediate WNT signaling in the presence of FGF and inhibited RA signaling

(A) Representative immunostaining of 36 h cultures of SOX2 (red), Brachyury (magenta), CDX2 (gray), and DAPI (blue). Scale bars, 100 μm.

(B) Boxplots showing mean gray value/nuclei quantified from repeat experiments as shown in (A). Each plot shows data points collected from two to four independent differentiations (>200 nuclei).

(C) Venn diagram showing the overlap of significantly upregulated genes in NMPs as reported in this study, Frith et al. (2018), and Verrier et al. (2018).

(D) Graph showing transcriptional fold change (FC) within the dataset of this study, of 26 genes commonly upregulated in NMPs according to the Venn diagram in (C).

(E) Venn diagram showing the overlap of upregulated HOX genes in NMPs as reported in this study and Frith et al. (2018).