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Identification of myeloid PPARα-deficient mice. (A,B) PCR analyses for Cre and Ppara allele in genomic DNA from Pparafl/fl and PparaMye−/− mice. (C) qPCR analysis for Ppara mRNA levels in macrophages or tissues from Pparafl/fl and PparaMye−/− mice (n=3–5). Data are expressed as means ± SD, n=3–5. ***, P<0.001. MEH, musculus epoxide hydrolase 1; WT, wild type; Mac, macrophage; WAT, white adipose tissue; BAT, brown adipose tissue; qPCR, quantitative real-time RT-PCR.
