Figure 4.

CD39, CD81, and CD130 expression reliably identifies human ASCs. (A, B) Representative histograms (left) and graph (right) showing the expression of the indicated surface molecules by ASCs versus other B cell subsets in human peripheral blood (A) and BM (B). ASCs and B cell subsets from peripheral blood and BM were gated as shown in Supplementary Figure 4A, B , respectively. Groups were compared using two-way ANOVA with the Geisser-Greenhouse correction followed by Dunnett’s multiple comparisons test. (C, D) FACS plots showing ASCs identification by the co-expression of CD39, CD81, CD130 or CD27 and CD20 in peripheral blood (C, left) and by the co-expression of CD39, CD81, CD130, CD326 or CD27 and CD38 in BM (D, top). (C) CD39⁺⁺CD20^low, CD81⁺CD20^low, CD130⁺CD20^low, and CD27⁺⁺CD20^low cells were gated on P4 ( Supplementary Figure 4A ). (D) CD38⁺⁺CD39⁺, CD38⁺⁺CD81⁺, CD38⁺⁺CD130⁺, CD38⁺⁺CD326⁺, and CD38⁺⁺CD27⁺⁺ cells were gated on P3 ( Supplementary Figure 4B ). Spearman correlation matrix showing reliable identification of ASCs populations in peripheral blood (C, right) and BM (D, bottom). Blue color coding indicates positive correlation with color intensity and the size of circle reflecting correlation coefficients. (E) Representative FACS plot showing the expression CD81 and CD130 by BM CD27⁺⁺CD38⁺⁺ PCs (red), CD27⁺CD38^- memory B cells (blue), CD27^-CD38⁺ (orange) and CD27^-CD38^- (green) immature B cells. Data were a compilation of 12 HDs and 6 SLE for peripheral blood, and 9 HDs for BM. Data show mean ± SEM (^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001). P values > 0.05 are not shown.