FIGURE 6.

Phenotyping of maize zmpp2c26 under drought stress. (A) Schematic diagram of Mu transposon in ZmPP2C26 gene and transcriptional detection ZmPP2C26 by RT-PCR. Black boxes represent exons; F and R, primer position in ZmPP2C26, and the sequences of them were listed in Supplementary Table 1. A 298 bp fragment of ZmPP2C26 was amplified from zmpp2c26 cDNA using F and R primers. A 306 bp fragment of ZmGAPDH was amplified and used as reference. W22, the wild type isolated from heterozygous zmpp2c26 mutant, was used as control. (B) Wilting phenotype of every line. The three-leaf stage seedlings were subjected to drought stress via withholding watering for 3 weeks, then re-watered for 5 days and photographed. (C) The survival rate of every line after treatment. (D) Root phenotype of every line. (E,F) Root length and root dry weight of every line. (G,H) Chlorophyll content and photosynthetic rate of every line before treatment. **P < 0.01.