Figure 3.

Detection of UCH-L1 and NF-L in brain and dorsal root ganglia (DRG) from nonclinical species

Wes capillary electrophoresis (ProteinSimple) was used to confirm cross-reactivity of the capture and detection antibodies within MSD R-PLEX kits for human UCH-L1 (F211O) and human NF-L (F217X). Each antibody was tested against brain homogenate (n = 4) from human, cynomolgus monkey, Wistar Han rat, and CD1 mouse. Dissected DRGs from monkey (n = 2) and rat (n = 4) were also assessed. Chemiluminescence signals were analyzed with Compass software (ProteinSimple), where image contrast was adjusted separately for each antibody to visualize the presence versus absence of the target peak.