Table 3.
Biomarker selection and prioritization based on reagent availability, cross-reactivity, and detection in dorsal root ganglia
| Biomarker | Cell type specificity | Reagent availability |
Cross-reactivity |
Detected in DRGs | |||
|---|---|---|---|---|---|---|---|
| MSD R-plex kit | Antibodies | Protein standard | Monkey | Rat | |||
| UCH-L1 | Neurons | F211O (human) | Included in kit | Included in kit | Yes | Yes | Yes |
| NF-L | Neurons (axons) | F217X (human) | Included in kit | Included in kit | Yes | Yes | Yes |
| IBA1 | Microglia | Not available | Abcam (ab178846) Abcam (ab178847) | Abcam (ab105593) | Yes | Yes | Yes |
| CNPase | Oligodendrocytes | Not available | Millipore (MAB326) CST (5664) |
Requires custom synthesis | Yes | Yes | Yes |
| MAP2 | Neurons (dendrites) | Not available | PhosphoSolutions (1099-MAP2) | Requires characterization & custom synthesis | Yes | Yes | Yes |
| GFAP | Astrocytes | F211M (human) | Included in kit | Included in kit | Yes | Yes | No |
| Tau (total) | Neurons (axons) | F218D (human) F228E (mouse) | Included in kit | Included in kit | Noa | Yesa | No |
a
For analysis of total Tau, monkey samples were tested using the human kit (F218D) and rat samples were tested using the mouse kit (F228E).