TABLE 1.

Epidemiology, phenotypes, and β-lactamase genes of K. pneumoniae isolates collected in 1994 and 1996

Isolate (yr collected)	Date collected (day/mo)	Ward	Source	Etest MIC ratioa	PFGE type	Plasmid size (kb)	β-Lactamase(s)
							Gene hybridizationb	pI value(s)	Sequence(s) found
79 (1994)	04/10	ICU	Central venous catheter	16	A	140	1 × TEM	5.6	TEM-63
						87	1 × SHV	6.3
								7.6	SHV-19
113 (1994)	No data	No data	Blood	>32	A	136	2 × TEM		TEM-53
						103	1 × SHV	5.6
						80		7.6	SHV-19
						63
114 (1994)	No data	No data	Blood	>32	A	136	2 × TEM		TEM-53
						103	1 × SHV	5.6
						80		7.6	SHV-19
						63
122c (1994)	09/10	ICU	Central venous catheter	<2	A	142	1 × TEM	5.4	TEM-1
						111	1 × SHV	7.6	SHV-19
						88
						72
						7
202c (1994)	12/10	ICU	Peritoneal fluid	>32	A1	186	1 × TEM	5.6	TEM-63
						111	1 × SHV	7.6	SHV-19
10 (1994)	27/09	Surgical	Pus	>32	A1	140	2 × TEM	5.6	TEM-63
						41	1 × SHV	6.3
								7.6	SHV-19
73 (1994)	02/10	Orthopedic	Pus	8	A2	140	1 × TEM	5.6	TEM-53
						41	1 × SHV	6.3
								7.6	SHV-19
91 (1994)	No data	No data	Blood culture	>32	A3	136	1 × TEM	5.6	TEM-63
						103	1 × SHV	7.6	SHV-19
						35
117c (1994)	05/10	ICU	Pus (abdomen)	<2	A3	103	1 × TEM		TEM-53
						80	1 × SHV	7.6	SHV-19
						63
						35
						30
118c (1994)	05/10	ICU	Pus (abdomen)	<2	C	136	1 × TEM		TEM-53
						103	1 × SHV	7.6	SHV-19
						80
						63
97 (1994)	No data	No data	Blood	<2	B	136	2 × TEM		TEM-1
						103	1 × SHV	7.6	SHV-19
571 (1994)	No data	No data	Blood	8	D	140	1 × TEM	5.4	TEM-1
						87	2 × SHV	5.6
						71		6.3
						59		6.8
						49		7.6	SHV-2 and -21
						41
						35
4291e (1996)	28/06	ICU	Endotracheal aspirate	64	A	139	3 × TEM		TEM-53
						88	2 × SHV	5.4
						50		5.6
								6.3
								6.8
								7.6	SHV-1 and -2
4495 (1996)	08/07	ICU	Endotracheal aspirate	>128	A4	139	3 × TEM	5.4	TEM-1 (2)f
						110	2 × SHV	5.6
						59		7.6	SHV-1
								8.2	SHV-5
4265d (1996)	27/06	ICU	Endotracheal aspirate	>128	E	142	2 × TEM	5.4	TEM-1 (2)
							2 × SHV	5.6
								6.3
								7.6	SHV-1
								8.2	SHV-5
4744d (1996)	18/07	Nursery	Sputum	>128	E	142	2 × TEM	5.4	TEM-1 (2)
							2 × SHV	6.3
								7.6
								8.2	SHV-5 (2)
8143d (1996)	29/06	ICU	Blood	>128	E	142	2 × TEM	5.4	TEM-1 (2)
							2 × SHV	5.6
								7.6
								8.2	SHV-5 and -22
4120 (1996)	20/06	ICU	Endotracheal aspirate	32	F	87	2 × TEM	5.4	TEM-1 (2)
						71	2 × SHV	6.3
						59		6.8
								7.6	SHV-2 (2)
4448 (1996)	05/07	Nursery	Pus (drip site)	64	F1	139	3 × TEM		TEM-53
						110	2 × SHV	5.4
						59		5.6
						50		7.6	SHV-19
								8.2	SHV-5
4175e (1996)	22/06	ICU	Endotracheal aspirate	4	G	140	1 × TEM	5.4	TEM-1
						87	2 × SHV	6.3
						7		6.8
						5		7.6	SHV-1 (2)
4183d (1996)	22/06	ICU	Endotracheal aspirate	>128	H	139	1 × TEM	5.4	TEM-1
						110	2 × SHV	5.6
						50		6.3
								7.6	SHV-20
								8.2	SHV-5
4634 (1996)	No data	No data	No data	>64	I	139	3 × TEM	5.4	TEM-1
						110	2 × SHV	6.8
						50		7.6	SHV-2 (2)
						23		8.2
4669 (1996)	17/07	Nursery	Pleural cavity tap	>32	J	139	3 × TEM	5.4	TEM-1 (3)
						110	2 × SHV	6.3
						72		7.6	SHV-2 and -20
						59		8.2
4700 (1996)	17/07	Pediatric	Urine	>64	K	186	2 × TEM	5.4
						111	2 × SHV	5.6	TEM-63 (2)
								6.3
								7.6	SHV-1 (2)
4824 (1996)	22/07	ICU	Endotracheal aspirate	8	L	142	2 × TEM	5.4	TEM-1 (2)
						59	2 × SHV	7.6	SHV-1 and -20
						7

^aRatio of MIC of ceftazidime to the MIC of ceftazidime plus clavulanate at 4 μg/ml, as determined by the Etest. 

^bNumbers of SHV genes were estimated from Southern blots of whole genomic DNA digested with SalI and hybridized with a SHV-specific probe, and the numbers of TEM genes were estimated from Southern blots of whole genomic DNA digested with BamHI, HindIII, or HincII and hybridized with a TEM-specific probe. 

^cFour isolates from patient I. 

^dFour isolates from patient II. 

^eTwo isolates from patient III. 

^fNumbers in parentheses indicate the number of copies of the gene encoding the β-lactamase indicated.