TABLE 3.

Nucleotide and amino acid sequence changes of TEM-63, SHV-19, SHV-20, SHV-21, and SHV-22^a

Enzyme	Nucleotide positionsb	Nucleotide change	Amino acid positionc	Amino acid change
TEM-63	555–57	CTT→TTT	21	Leu→Phe
	304–306	GAG→AAG	104	Glu→Lys
	484–486	CGT→AGT	164	Arg→Ser
	538–540	ATG→ACG	182	Met→Thr
SHV-19	505–507	CTT→TTT	173	Leu→Phe
SHV-20	505–507	CTT→TTT	173	Leu→Phe
	700–702	GGC→AGC	238	Gly→Ser
SHV-21	352–354	CTC→TTC	122	Leu→Phe
	505–507	CTT→TTT	173	Leu→Phe
	700–702	GGC→AGC	238	Gly→Ser
SHV-22	460–462	AAC→AAG	158	Asn→Lys
	700–702	GGC→AGC	238	Gly→Ser
	703–705	GAG→AAG	240	Glu→Lys

^aAbbreviations: A, adenine; C, cytosine; G, guanine; T, thymine; Ala, alanine; Asn, asparagine; Glu, glutamate; Leu, leucine; Lys, lysine; Phe, phenylalanine; Ser, serine; Gly, glycine. 

^bNucleotide numbering is in accordance with the AGT codon start of previously reported SHV sequences. 

^cAmino acid numbering is according to Ambler et al. (1).