Figure 3.

The Ab-VAMP₇₇ polyclonal antibody detects VAMP cleavage by BoNT/B at the neuromuscular junction. (A) Scheme showing the staining of the two LAL muscles collected after injection of either saline or BoNT/B (top panel) or of either BoNT/D or BoNT/G (bottom panel) and the following immunostaining of one LAL with an antibody against intact VAMP-1 (top panels) or with the Ab-VAMP₇₇ antibody (bottom panels). In both cases, the muscles were also stained for the vesicular protein marker v-AchT. (B–E) Representative images of whole-mount LAL muscles injected with either saline (B) or BoNT/B (C) or BoNT/D (D) or BoNT/G (E) stained for intact VAMP-1 (red in top panels) or cleaved VAMP (red in bottom panels). The right panels of each condition show the merge with v-AchT (green). Scale bar = 25 μm.