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The Ab-VAMP77 polyclonal antibody detects VAMP cleavage by TeNT in the brain stem. The TeNT was injected either at the level of the LAL in the mouse (A) or at the level of the whisker pad in rats (B) and then the cleavage of VAMP was evaluated in the brain stem with an antibody against intact VAMP-2 (green) and Ab-VAMP77 (red). The bottom panels in (A,B) show a magnification of the white-squared area. Scale bar = 40 μm.
