Table 2.

Treatment media preparation. CSM is the positive control for all experiments. The treatments 0 mM nitrogen and 0 mM K₂HPO₄ are the negative controls for each of the nutrient concentration series.

	CSM	Starvation CSM	Organic Nitrogen (L-Ala/L-Met)	0 mM Nitrogen	NH4Cl Concentration Series	NaNO3 Concentration Series	0 mM K2HPO4	K2HPO4 Concentration Series
MgSO4·7H2O (mM)	1.66	1.66	1.66	1.66	1.66	1.66	1.66	1.66
CaCl2 (mM)	0.033	0.033	0.033	0.033	0.033	0.033	0.033	0.033
NH4NO3 (mM)	2.5	0	0	0	0	0	2.5	2.5
NH4Cl (mM)	0	0	0	0	1.5–50 1	0	0	0
NaNO3 (mM)	0	0	0	0	0	1.5–50 1	0	0
D-glucose (mM)	22	22	22	22	22	22	22	22
L-alanine (mM)	10.10	0	10.10	0	0	0	10.10	10.10
L-methionine (mM)	0.67	0	0.67	0	0	0	0.67	0.67
K2HPO4 (mM)	3.44	0	3.44	3.44	3.44	3.44	0	1.5–20 2
FeEDTA (μM)	1.36	1.36	1.36	1.36	1.36	1.36	1.36	1.36
Trace elements (mL) 3	2.5	2.5	2.5	2.5	2.5	2.5	2.5	2.5
DI H2O 4 (mL)	997.5	997.5	997.5	997.5	997.5	997.5	997.5	997.5

¹ 1.5, 3, 5, 10, 20, 50 mM series used for liquid media; ² 5, 20, 50 mM concentration series used for solid media where agar (2% w/v) is added; ³ Trace element stock contains 10 μM MnCl₂·4H₂0, 10 μM ZnCl₂, 33 μM H₃BO₃, 1 μM CuSO₄.5H₂O and 0.2 μM Na₂MoO₄·2H₂O; ⁴ Deionised water (DI H₂O).