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. 2022 Apr 11;19(8):4564. doi: 10.3390/ijerph19084564

Figure 2.

Figure 2

FLC induced apoptosis and altered up-regulated the level of pro-apoptotic proteins. TM4 cells were treated by FLC at the concentrations of 0, 40, 80, 160 μM for 6, 12, 24h. Apoptotic cells (6 h) were detected by flow cytometry and apoptosis protein levels (6, 12, 24 h) were determined by Western Blot with indicated antibodies. (a) The apoptotic cells were detected by Annexin V-PI double staining using Flow cytometer; (b) The quantitative data was analyzed by FlowJo and GraphPad Prism software (n = 3); (c) The representative blots were shown; (d) The quantitative data was analyzed by ImageJ and GraphPad Prism software (n = 6). * p < 0.05, ** p < 0.01,**** p < 0.0001 vs. control group (0 μM FLC).