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. 2022 Apr 11;19(8):4564. doi: 10.3390/ijerph19084564

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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ISRIB alleviated FLC-induced cytotoxicity and apoptosis. TM4 cells were treated by ISRIB at the concentration of 200 and 400 nM for 6 h before treated by FLC at the concentration of 160 μM for 6 h. (a) Cell viability was detected by CCK-8 assay (n = 6); (b) Cytotoxicity was detected by LDH leakage assay (n = 6). Apoptotic cells were determined by flow cytometry; (c) The representative images were shown; (d) The quantitative data was analyzed by FlowJo and GraphPad Prism software (n = 3). * p < 0.05, *** p < 0.001, **** p < 0.0001 vs. corresponding group.

ISRIB alleviated FLC-induced cytotoxicity and apoptosis. TM4 cells were treated by ISRIB at the concentration of 200 and 400 nM for 6 h before treated by FLC at the concentration of 160 μM for 6 h. (a) Cell viability was detected by CCK-8 assay (n = 6); (b) Cytotoxicity was detected by LDH leakage assay (n = 6). Apoptotic cells were determined by flow cytometry; (c) The representative images were shown; (d) The quantitative data was analyzed by FlowJo and GraphPad Prism software (n = 3). * p < 0.05, *** p < 0.001, **** p < 0.0001 vs. corresponding group.