PFGE analysis of genomic DNAs from N. gonorrhoeae strains digested with SpeI and NheI. Lanes 1, 11, and 20, lambda DNA ladder; lanes 2 to 10 and 12 to 13 (after SpeI digestion), strains 80/96, 88/96, 121/95, 128/95, 160/95, 165/95, 31/95, 253/95, 109/96, 134/97, and 105/98, respectively; lanes 14 to 19 (after NheI digestion), strains 80/96, 88/96, 121/95, 128/95, 160/95, and 165/95, respectively. (See Table 2 for epidemiologic data.)