FIG. 1.

(A) Viable A. fumigatus showing green fluorescent hyphae with clearly red fluorescent vacuolar structures. (B) A. fumigatus hyphae treated with 2 μg of voriconazole per ml for 9 h. Impairment is shown by green fluorescent hyphae and a lack of red fluorescent vacuolar structures. (C) A. fumigatus hyphae treated with 8 μg of voriconazole per ml for 48 h. Clusters of collapsed hyphae with extremely bright, diffuse, green-yellow fluorescence without vacuolar structures are displayed by dead fungi. (D) A. fumigatus hyphae treated with 16 μg of voriconazole per ml for 48 h. Only one fragment of hyphae is apparently vital.