TABLE 2.

Susceptibility of Aspergillus hyphae to voriconazole and itraconazole

Aspergillus sp.a (no. of isolates tested)	Voriconazole MFC range (μg/ml) at 48 h	Voriconazole staining pattern(s)b	Itraconazole MFC range (μg/ml) at 48 h	Itraconazole staining pattern(s)
A. flavus (5)	8–>16	VID	4–4	ID
	2–2	D	2–2	D
	2–4	D	2–2	D
	8–>16	VD	4–4	D
	2–4	D	4–8	D
A. terreus (5)	2–4	D	4–4	D
	2–4	ID	4–4	ID
	16	VD	2–2	D
	2–4	D	8–>16	VD
	2–2	D	4–4	D
A. niger (5)	8–16	ID	8–>16	VD
	4–4	D	4–4	D
	2–2	D	8–>16	VD
	2–2	D	2–2	D
	2–2	D	2–2	D
A. fumigatus (5)	2–2	D	2–2	D
	2–4	ID	2–4	ID
	8–>16	VID	4–4	ID
	4–4	D	2–2	D
	2–2	D	2–4	D
Itraconazole-resistant A. fumigatus (1)	2–2	D	>16	VID

^aEach isolate was tested three times. 

^bV, Viable hyphae represented by green fluorescent hyphae with red vacuolar structures; I, impaired hyphae represented by green fluorescent hyphae and lack of red fluorescent vacuolar structures; D, dead hyphae represented by green-yellow fluorescence without any vacuolar structures. The predominant patterns are underlined.