FIG. 4.

Growth and bioluminescence curves of EC14(pCGLS1.UC) grown in vivo using the neutropenic-mouse thigh model of infection. Viable counts are reported as CFU per thigh, and bioluminescence is represented as PCs as measured using the ICCD camera. Each data point is the mean ± SE determined using two animals, with the exception of the 12-h time point, which is the mean ± SE of four animals. Bioluminescence was determined at each time point from a set of two or four animals immediately prior to determination of viable-cell count. In this study, the correlation of viable-cell count to bioluminescence was 0.95 from 4 to 12 h and 0.88 from 2 to 12 h. IC, infection control; CAZ, ceftazidime treatment (50 mg/kg at 1 and 5 h postinfection).