Figure 4.

Terazosin diminishes S. Typhimurium intracellular replication in raw macrophage cells. (A) Microscopic images of S. Typhimurium in macrophages. Norepinephrine markedly increased the number of intracellularly replicating bacterial cells and terazosin at the sub-MIC diminished the bacterial intracellular replication in comparison to untreated S. Typhimurium. (B) The infected macrophages were lysed, and the phagocytosed cell/relative untaken cell percentage and x-fold intracellular replication were calculated. The experiment was repeated in triplicate, and the data are presented as the mean ± the SD. A one-way ANOVA test, followed by Tukey’s multiple comparison post-hoc test, was employed to attest the statistical significance; p < 0.05 was considered significant. *** = p < 0.0001; ** = p < 0.001; * = p < 0.05. Scale bars correspond to 100 μm.