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. 2022 Apr 7;12(4):549. doi: 10.3390/life12040549

Figure 2.

Figure 2

Adhesion and migration of ASCs cultured in CM from bladder cancer cells. (A) The migration of ASCs cultured in CM from 5637 and HB-CLS-1 cells decreased by 24.0% and 32.4%, respectively. No differences were observed for ASCs cultured in CM from HT-1376 cells. (B) Soluble mediators secreted by 5637 and HB-CLS-1 cells reduced sheet migration. After 24 h observation, 87.4% and 65.3% of the scratch wounds closed compared to 95.7% for cells cultured in a standard growth medium. No significant changes in growth kinetics were observed for ASCs cultured in CM from HT-1376 cells. (C) ASC adhesion significantly increased after culture in conditioned medium from bladder cancer cell lines. Depending on the ECM component, cell adhesion rose from 36.3% to 65.4% for ASCs cultured in CM from 5637 cells and from 56.1% to 83.0% for ASCs cultured in CM from HB-CLS-1 cells. (D) Culture with soluble mediators secreted by HT-1376 cells reduced ERK and AKT activation in ASCs by 37.4% and 18.8%, respectively. No differences were noted after culture in CM from 5637 cells. Although not statistically significant, decreased activation of P70 S6K was observed after culture in CM from all three bladder cancer cell lines. Red/orange stands for 5637 cells, gray for HB-CLS-1, and yellow for HT-1376 cells. All results are presented as a percentage of control. Bars represent standard deviation; * p > 0.05, ** p > 0.01, *** p > 0.001.