Table 7.
Total lipid content, cholesterol content, and fatty-acid (FA) composition in the breast meat of broilers (n = 10).
| Dietary Treatments 1 | SEM 2 | p-Value | ||||
|---|---|---|---|---|---|---|
| Item | Control | LA | LAR | LAE | ||
| Total lipids (g/100 g) | 1.69 a | 1.25 b | 1.25 b | 1.23 b | 0.093 | 0.003 |
| Cholesterol (mg/g) | 0.463 | 0.561 | 0.569 | 0.547 | 0.0443 | 0.318 |
| FA composition (g/100 g FA) | ||||||
| 10:0 | 0.001 | 0.002 | 0.006 | 0.004 | 0.0020 | 0.441 |
| 12:0 | 0.036 | 0.037 | 0.036 | 0.029 | 0.0028 | 0.208 |
| 14:0 | 0.29 a | 0.23 b | 0.25 a,b | 0.22 b | 0.011 | 0.001 |
| 14:1c9 | 0.023 a | 0.007 b | 0.006 b | 0.002 b | 0.0043 | 0.006 |
| 15:0 | 0.071 | 0.082 | 0.080 | 0.072 | 0.0044 | 0.236 |
| 16:0 | 15.3 a | 13.9 b | 13.9 b | 13.7 b | 0.24 | <0.001 |
| 16:1c7 | 0.34 a | 0.26 b | 0.28 b | 0.26 b | 0.014 | <0.001 |
| 16:1c9 | 0.92 a | 0.67 b | 0.69 a,b | 0.66 b | 0.062 | 0.014 |
| 17:0 | 0.19 | 0.20 | 0.19 | 0.19 | 0.011 | 0.874 |
| 17:1c9 | 0.039 | 0.024 | 0.035 | 0.024 | 0.0048 | 0.083 |
| 18:0 | 8.57 | 9.14 | 8.69 | 9.18 | 0.394 | 0.609 |
| 18:1c9 | 25.6 | 23.1 | 24.5 | 23.6 | 0.65 | 0.052 |
| 18:1c11 | 1.21 | 1.42 | 1.42 | 1.39 | 0.059 | 0.049 |
| 18:2n-6 | 36.7 | 37.8 | 38.4 | 37.6 | 0.88 | 0.575 |
| 18:3n-6 | 0.14 b | 0.15 a,b | 0.16 a | 0.16 a | 0.004 | 0.001 |
| 18:2t9t12 | 0.29 a | 0.25 a,b | 0.24 b | 0.24 b | 0.015 | 0.027 |
| 18:3n-3 | 0.55 a | 0.41 b | 0.40 b | 0.42 b | 0.025 | <0.001 |
| 18:4n-3 | 0.004 | 0.015 | 0.068 | 0.004 | 0.0246 | 0.218 |
| 20:0 | 0.094 | 0.090 | 0.094 | 0.090 | 0.0032 | 0.670 |
| 20:1c11 | 0.23 a | 0.19 b | 0.20 b | 0.20 b | 0.007 | 0.001 |
| 20:2n-6 | 0.56 | 0.62 | 0.56 | 0.59 | 0.036 | 0.637 |
| 20:3n-6 | 0.63 a | 0.56 a,b | 0.48 b | 0.55 a,b | 0.029 | 0.012 |
| 20:4n-6 | 4.59 | 6.01 | 6.08 | 5.16 | 0.507 | 0.132 |
| 20:3n-3 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| 20:5n-3 | 0.025 b | 0.097 a | 0.108 a | 0.095 a | 0.0076 | <0.001 |
| 22:0 | 0.039 | 0.027 | 0.040 | 0.037 | 0.0077 | 0.615 |
| 22:1n-9 | 0.004 | 0.006 | 0.024 | 0.000 | 0.0095 | 0.308 |
| 22:2n-6 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| 22:5n-3 | 0.21 b | 0.58 a | 0.46 a | 0.57 a | 0.049 | <0.001 |
| 22:6n-3 | 0.12 b | 0.47 a | 0.32 a,b | 0.49 a | 0.065 | 0.001 |
| Others | 3.23 | 3.60 | 3.23 | 3.46 | 0.332 | 0.820 |
| Partial sums of FA, g/100 g FA | ||||||
| SFA | 24.6 | 23.8 | 23.2 | 23.6 | 0.58 | 0.433 |
| cis-MUFA | 28.4 a | 25.8 b | 27.1 a,b | 26.2 a,b | 0.68 | 0.045 |
| PUFA | 43.8 b | 46.9 a | 46.4 a | 46.8 a | 0.48 | <0.001 |
| n-6 PUFA | 42.6 b | 45.1 a | 44.8 a | 45.0 a | 0.50 | 0.003 |
| n-3 PUFA | 0.90 b | 1.57 a | 1.38 a | 1.57 a | 0.101 | <0.001 |
| Ratios of FA | ||||||
| PUFA/SFA | 1.79 b | 2.00 a | 2.01 a | 2.00 a | 0.062 | 0.046 |
| n-6/n-3 | 47.7 a | 30.2 b | 34.4 b | 29.6b | <0.001 | |
1 Control, corn–soybean basal diet; LA, basal diet plus 15% L. digitata; LAR, basal diet plus 15% L. digitata + 0.005% Rovabio® Excel AP; LAE, basal diet plus 15% L. digitata + 0.01% recombinant CAZyme. 2 SEM, standard error of the mean. SFA = sum of (10:0, 12:0, 14:0, 15:0, 16:0, 17:0, 18:0, 20:0, 22:0). cis-MUFA = sum of (14:1c9, 16:1c7, 16:1c9, 17:1c9, 18:1c9, 18:1c11, 20:1c11, 22:1n-9). PUFA = sum of (18:2n-6, 18:2t9t12, 18:3n-6, 18:3n-3, 18:4n-3, 20:2n-6, 20:3n-6, 20:4n-6, 20:3n-3, 20:5n-3, 22:5n-3, 22:6n-3). n-6 PUFA = sum of (18:2n-6, 18:3n-6, 20:2n-6, 20:3n-6, 20:4n-6). n-3 PUFA = sum of (18:3n-3, 18:4n-3, 20:3n-3, 20:5n-3, 22:5n-3, 22:6n-3). a,b Different superscript letters within a row indicate a significant difference (p < 0.05). n.d., not detected.