Table 8.
Total lipid content, cholesterol content, and fatty-acid (FA) composition in the thigh meat of broilers (n = 10).
| Dietary Treatments 1 | SEM 2 | p-Value | ||||
|---|---|---|---|---|---|---|
| Item | Control | LA | LAR | LAE | ||
| Total lipids (g/100 g) | 3.35 a | 2.36 b | 2.56 b | 2.22 b | 0.134 | <0.001 |
| Cholesterol (mg/g) | 0.664 | 0.611 | 0.657 | 0.571 | 0.0376 | 0.280 |
| FA composition (g/100 g FA) | ||||||
| 10:0 | 0.004 | 0.009 | 0.009 | 0.005 | 0.0020 | 0.103 |
| 12:0 | 0.042 | 0.038 | 0.044 | 0.038 | 0.0023 | 0.159 |
| 14:0 | 0.33 a | 0.27 b | 0.29 b | 0.28 b | 0.010 | 0.002 |
| 14:1c9 | 0.035 a | 0.024 b | 0.025 b | 0.029 a,b | 0.0024 | 0.014 |
| 15:0 | 0.077 | 0.074 | 0.081 | 0.083 | 0.0026 | 0.092 |
| 16:0 | 15.6 a | 13.6 b | 14.2 b | 13.5 b | 0.27 | <0.001 |
| 16:1c7 | 0.41 a | 0.32 b | 0.34 b | 0.36 a,b | 0.013 | <0.001 |
| 16:1c9 | 1.43 a | 1.10 b | 1.15 a,b | 1.19 a,b | 0.081 | 0.033 |
| 17:0 | 0.17 | 0.17 | 0.18 | 0.17 | 0.006 | 0.850 |
| 17:1c9 | 0.045 | 0.036 | 0.040 | 0.039 | 0.0021 | 0.093 |
| 18:0 | 7.38 | 8.02 | 7.73 | 7.40 | 0.315 | 0.437 |
| 18:1c9 | 29.3 | 27.4 | 28.9 | 28.5 | 0.51 | 0.063 |
| 18:1c11 | 1.12 b | 1.33 a | 1.30 a | 1.26 a | 0.037 | 0.001 |
| 18:2n-6 | 37.5 | 39.9 | 38.6 | 39.9 | 0.82 | 0.134 |
| 18:3n-6 | 0.10 | 0.11 | 0.11 | 0.11 | 0.004 | 0.755 |
| 18:2t9t12 | 0.28 a | 0.21 b | 0.18 b | 0.22 a,b | 0.017 | 0.003 |
| 18:3n-3 | 0.58 a | 0.45 b | 0.45 b | 0.46 b | 0.027 | 0.002 |
| 18:4n-3 | 0.017 | 0.020 | 0.021 | 0.022 | 0.0034 | 0.770 |
| 20:0 | 0.09 | 0.10 | 0.10 | 0.10 | 0.005 | 0.589 |
| 20:1c11 | 0.23 | 0.22 | 0.23 | 0.21 | 0.006 | 0.060 |
| 20:2n-6 | 0.36 | 0.38 | 0.35 | 0.35 | 0.013 | 0.294 |
| 20:3n-6 | 0.42 a | 0.34 b | 0.31 b | 0.32 b | 0.018 | 0.001 |
| 20:4n-6 | 2.14 b | 3.04 a | 2.55 a,b | 2.70 a,b | 0.168 | 0.006 |
| 20:3n-3 | 0.014 a | 0.008 a,b | 0.001 b | 0.002 a,b | 0.0032 | 0.032 |
| 20:5n-3 | 0.013 b | 0.046 a | 0.036 a | 0.041 a | 0.0037 | <0.001 |
| 22:0 | 0.040 | 0.046 | 0.047 | 0.046 | 0.0024 | 0.176 |
| 22:1n-9 | 0.058 | 0.062 | 0.061 | 0.048 | 0.0147 | 0.889 |
| 22:2n-6 | 0.008 | 0.009 | 0.006 | 0.008 | 0.0019 | 0.726 |
| 22:5n-3 | 0.10 b | 0.29 a | 0.21 a | 0.23 a | 0.019 | <0.001 |
| 22:6n-3 | 0.09 b | 0.30 a | 0.23 a | 0.26 a | 0.026 | <0.001 |
| Others | 2.07 | 2.17 | 2.16 | 2.09 | 0.104 | 0.879 |
| Partial sums of FA, g/100 g FA | ||||||
| SFA | 23.7 | 22.3 | 22.7 | 21.7 | 0.51 | 0.052 |
| cis-MUFA | 31.2 | 29.4 | 30.9 | 30.4 | 0.53 | 0.095 |
| PUFA | 41.6 | 45.1 | 43.1 | 44.7 | 0.90 | 0.041 |
| n-6 PUFA | 40.5 | 43.7 | 41.9 | 43.4 | 0.87 | 0.053 |
| n-3 PUFA | 0.81 c | 1.11 a | 0.95 b,c | 1.02 a,b | 0.037 | <0.001 |
| Ratios of FA | ||||||
| PUFA/SFA | 1.76 b | 2.04 a,b | 1.92 a,b | 2.07 a | 0.079 | 0.040 |
| n-6/n-3 | 50.1 a | 40.0 c | 44.4 b | 42.7 b,c | 1.10 | <0.001 |
1 Control, corn–soybean basal diet; LA, basal diet plus 15% L. digitata; LAR, basal diet plus 15% L. digitata + 0.005% Rovabio® Excel AP; LAE, basal diet plus 15% L. digitata + 0.01% recombinant CAZyme. 2 SEM, standard error of the mean. SFA = sum of (10:0, 12:0, 14:0, 15:0, 16:0, 17:0, 18:0, 20:0, 22:0). cis-MUFA = sum of (14:1c9, 16:1c7, 16:1c9, 17:1c9, 18:1c9, 18:1c11, 20:1c11, 22:1n-9). PUFA = sum of (18:2n-6, 18:2t9t12, 18:3n-6, 18:3n-3, 18:4n-3, 20:2n-6, 20:3n-6, 20:4n-6, 20:3n-3, 20:5n-3, 22:5n-3, 22:6n-3). n-6 PUFA = sum of (18:2n-6, 18:3n-6, 20:2n-6, 20:3n-6, 20:4n-6). n-3 PUFA = sum of (18:3n-3, 18:4n-3, 20:3n-3, 20:5n-3, 22:5n-3, 22:6n-3). a,b,c Different superscript letters within a row indicate a significant difference (p < 0.05). n.d., not detected.