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. 2022 Apr 12;11(8):1052. doi: 10.3390/plants11081052

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a,b) show the CRISPR/Cas9 Agrobacterium transformation of wheat by integrating it with speed breeding to reduce the generation time. Blue and red color shows the transformation with GRF–GIF and without it, respectively. This procedure consists of different steps: (1,2) Screening of best lines and collection of germplasm, (3) embryo collection under a microscope, (4) vector construction and Agrobacterium transformation (blue with GRF–GIF and red without it), (5) Auxin callus induction, more in (a,b), (6) antibiotic selection (do not need in GRF–GIF method), (7) integration of speed breeding with the CRISPR/Cas9.