Figure 2.

Colocalization of amylin protein with neuron-specific markers in the immature rat brain. (A) Amylin protein was co-expressed with neuron-specific markers such as NeuN, a marker of mature neurons; TH, a marker of dopaminergic neurons; IBA1, a marker of microglia; olig2, a marker of oligodendrocytes; and GFAP, a marker of astrocytes. Amylin protein was colocalized with mature neurons in the cerebral cortex and dopaminergic neurons in the VTA. (B) Quantification of colocalization in the immature rat brain. Colocalization was shown in yellow in the merged image. Colocalization analysis was performed using ImageJ software. Scale bar, 50 μm.