Figure 2.

KCa3.1 channels regulate macrophage phenotype (A) Modulation of CD206 expression by infiltrating macrophages, identified as CD45⁺CD11b⁺MHCII⁺F4/80⁺ mononucleated cells from hindlimb muscles of 8 weeks old mice. Top panel: representative example of CD206 flow cytometric profile. FMO = fluorescence minus one as negative control; V1 = vehicle-treated animal 1; T2 = TRAM-34-treated animal 2. Bottom panel: median fluorescence intensity of CD206 expression from 3 vehicle-treated (V) and 4 TRAM-34 treated (T) animals. Dots represent individual animals; bars represent the mean value ± SD. P obtained by Student’s t-test. (B) Relative expression level of Arg1, CD2016 and iNOS genes in the diaphragm of TRAM-34-treated mdx mice (age, 8 weeks), compared to each gene in vehicle-treated mice (dotted line). Bars: mean ± SD, dots, individual values in 4 experimental groups. A total of 6 vehicle-treated and 7 TRAM-34-treated animals was examined. *: significantly different from 1 (p = 0.016). (C) The fraction of arginase-expressing cells is increased in the diaphragm of mdx mice treated with TRAM-34 (T) as compared to vehicle (V), independent of animal age. Top panels: representative images. Note the increase in the density of regions Iba1⁺–Arg1⁺ (in yellow); scale bars: 50 μm. Bottom panel: mean ± SD value (bars), and individual data (white circles). N = 10 (3–8 weeks), 6 (6–9 and 15–19 weeks).