Figure 3.

Expression of angiogenesis-related genes in distal samples. (A–C) Quantitative analysis of expression of the genes investigated in samples of the distal great saphenous vein or other vein -according to groups- (tissue obtained during surgery) was performed by quantitative RT-PCR. Relative expression of Eng, endoglin (A), HIF1A: hypoxia-inducible factor 1A (B) and VEGFA: vascular endothelial growth factor A (C) in recurrent varicose vein vs primary varicose vein were analysed. For data normalisation, qPCR analyses are represented as logarithmic transformation of the relative expression; box plots show median and 25–75 percentiles, and whiskers show 10–90 percentile. ns: Not significant.