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Western blots of patient PBMCs (A) freshly isolated from blood, and (B) 7 days after culturing the PBMCs in RPMI. uT (untreated) designates PBMCs isolated from blood taken before the patient was treated with Abatacept while Tr (treated) designates PBMC isolated from blood taken after the patient was treated with Abatacept. GAPDH was used as a loading control.
