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siRNA-mediated knockdown of LRBA. MIN6 cells were transfected for 72 h with siRNA targeting LRBA. (A) The Western blot shows successful knockdown of LRBA (at ≈319 kDa). Β-actin was used as a loading control (at ≈43 kDa). (B) qRT-PCR quantification of LRBA mRNA in MIN6 cells after 72 h of transfection yielded a knockdown efficiency of ≈60%, respectively. Error bars represent SEM of three independent experiments.
