Figure 4.
The stability of rPA83m (a), non-modified rPA83 (b), rPA3+4 and rPA1+2 (c); (a)—1, 2—rPA83m not exposed to incubation. 3–6—four independent replicates of a rPA83m sample incubated at +4 °C for 144 days. (b)—1—non-modified rPA83 not exposed to incubation, 2–7—non-modified rPA83 incubated at +4 °C for 1, 4, 7, 14, 33 and 55 days, respectively. (c)—L—protein molecular weight markers ladder (molecular weights, in kDa, are indicated on the left). 1, 4—rPA3+4 not exposed to incubation. 2, 3—rPA3+4 incubated at +25 °C for 17 and 160 days, respectively. 5, 6—rPA3+4 incubated at +37 °C for 21 and 160 days, respectively. 7—rPA1+2 not exposed to incubation. 8—rPA1+2 incubated at +25 °C for 160 days. 9—rPA1+2 incubated at +37 °C for 160 days. Original gels are presented in the Figure S2.
