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. 2022 Apr 6;14(4):806. doi: 10.3390/pharmaceutics14040806

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Simultaneous adsorption of rPA1+2 and rPA3+4 to SPs. (a–c)—Fluorescence microscopy of rPA1+2 labelled with fluorescein isothiocyanate (rPA1+2^FITC) and rPA3+4 labelled with rhodamine isothiocyanate (rPA3+4^RITC) simultaneously adsorbed to SPs. Images are presented in FITC fluorescence channel (a), RITC fluorescence channel (b) and phase contrast mode (c). Images of control samples (SPs compositions with rPA1+2^FITC or rPA3+4^RITC separately and uncoated SPs) fluorescence analysis are presented in the Figure S4. (d,e)—Electrophoresis analysis of modified recombinant anthrax antigens labelled with fluorophores in 8–20% SDS-PAGE. 1—rPA3+4^RITC; 2—rPA1+2^FITC; 3—Compositions of rPA3+4^RITC and rPA1+2^FITC with SPs. Unstained gel under UV-light (d) and Coomassie blue G-250 staining (e). The SPs/rPA1+2^FITC/rPA 3+4^RITC mass ratio within Compositions was 20/1/1. Compositions were obtained in 1xPBS. Original gel is presented in the Figure S5.